Meso Scale Discovery multiplex assay helps scientists detect biomarkers and other analytes in single and multiplex assay formats. Meso Scale Discovery assays combine electrochemiluminescence detection and multiarray technology that delivers highly dense and rapid experimental data through the organization, miniaturization, and parallel processing of study samples. Besides, the MSD multiplex ELISA product line includes a broad range of assay types, such as MSD cytokine and PK assays, as well as a plethora of reagents and assay plates. The current article discusses the superiority of Meso Scale Discovery assays such as MSD cytokine multiplex and other MSD multiplexed ELISA assays for immunogenicity assessments and other drug development studies.
The power of MSD multiplex ELISA assays
Multiplexing is a tool that allows researchers to quantify multiple analytes within a single assay volume. This feature saves time and resources. Today, multiplexing is an invaluable tool for assessing studies, such as toxicity studies, biomarker discovery assays, and mechanism of action assessments.
The Meso Scale Discovery assay provides performance and convenience in singleplex and multiplex platforms to quantify multiple analytes in a single assay volume. This characteristic saves costs and experimental time and preserves precious biological specimens. The standard 96-well multi-spot MSD multiplex assay plate can quantify up to 10 different analytes in less than 25 microliters of sample. Additionally, the 384-well format can quantify up to four different analytes, delivering 1536 data points per experimental run. Some of the primary benefits of Meso Scale Discovery multiplex assays are:
- Rapid processing of study samples
- Running of 10 assays in a single plate
- Efficient use of samples where researchers can use less than one microlitre per well
- Hustle free instrumentation
- Customization capacities for designing specific study panels
Moreover, MSD assays are extremely sensitive and can deliver excellent quantification results from diluted as well as neat samples.
Read: Why ELISA to MSD Conversion is Vital for Advancing Drug Development
Types of Meso Scale Discovery multiplex assays
The Meso Scale Discovery platform offers two solutions: V-plex and U-plex assays. V-plex MSD assays are validated systems that can be directly used in an experimental setup. These panels are preconfigured, but researchers can customize these panels by selecting from a wide range of analytes. Once analytes are selected, MSD then validates these panels for research use. All V-plex assays undergo rigorous validation and are supported by a certificate of analysis. The validation of V-plex assay kits includes:
- Percent recovery and coefficient of variance
- The lower limit of quantification and upper limit of quantification
- The extent of nonspecific binding
- Standard curve data
- Full GMP and GLP validations
On the other hand, U-plex multiplex assays are customized systems that are not prevalidated and require experimental preparation. The U stands for you as a user. This assay system requires researchers to bind biotin-streptavidin linkage with the protein but facilitates antibody combinations un available with the V-plex kits. Hence, any combination of analytes can be studied using the U-plex assay platform. As researchers must design the system, Meso Scale Discovery offers over 75 possible biotin-labeled antibodies for U-plex multiplex assays.
Therefore, MSD multiplex ELISA assays offer superior advantages over traditional bioanalytical platforms.
